2 results for “Separation”
SDS-Page Analysis Of Cysticercus tenuicollis In Vitro And In Vivo In Basrah Province/ Southern Iraq
The larval stage Cysticercus tenuicollis was isolated from slaughtered sheep and goats at the Basrah slaughterhouse with 30 samples. In vitro 16 samples, 2 control, and 14 were treated with plant extracts and novel chemical compounds. In vivo 14 sample,1 control and 13 treated with plant extracts and novel chemical compounds.. SDS- page electrophoresis using the gel as separation gel of larval stage Cysticercus tenuicollis. Lanes (C) control, (A) Syzygium aromaticum, (B) Capparis spinosa, novel acylselenourea, i.e., 4-Nitro-N-((4-(N-(pyrimidin-2-yl) sulfamoyl) phenyl) carbamoselenoyl) Benz amide (1), 4-Methyl-N-((4-(N-(pyrimidin-2-yl) sulfamoyl) phenyl) carbamoselenoyl) Benz amide (2), 4-Nitro-N-((4-nitrophenyl) carbamo selenoyl) Benz amide (4), N-(2,6-dioxo-1,2,3,6-tetrahydropyrimidine-1-carbonoselenoyl)-4-nitrobenzamide (5) and novel acylthiourea 4-Methyl-N-((4-(N-(pyrimidin-2-yl) sulfamoyl phenyl) carbamothioyl) Benz amide (3). The rats were administered plant extracts and synthesis compounds orally via mouth for 15 days, Then the rats were dissected, the cysts were isolated, and their SDS page was measured.The results found in in vitro, all plant extracts, and synthesized compounds bands ranged from 67 to 17KD. In contrast, the protein bands of cysts varied from 67 to 17KD in vivo whereas Capparis spinosa and the synthetic chemical (5) showed no bands. This study recorded a clear effect on protein cotenants of C. tenuicollis which was treated with two plant extracts C. spinosa and S. aromaticum, and five novel chemical synthesized compounds in vitro and in vivo by SDS- PAGE as compared with untreated.
Liquid Biphasic System : A Review on Modern Bioseparation Technologies
Many researchers have been interested in a substitute bioseparation technology for different biomolecules, namely the liquid biphasic system (L.B.S.). The analysis starts with a detailed discussion of the basic concept of L.B.S. and follows by a debate on the further creation of the different components for phase-forming of L.B.S. Also, various advance L.B.S. technologies will be introduced. It was addressed that is beneficial to L.B.S.'s e-function for bimolecular extraction, separation, and purification. The L.B.S. key parameters for link were obtainable and assessed. Furthermore, a guideline for future growth was described as future opportunities and challenges of L.B.S. The measures outlined in this review gives an insight into potential studies of the techniques of liquid-liquid split.