Pharmacy and Applied Health Sciences

Development of Polymeric Nanoparticles of Sunitinib malate in Eudragit S 100 for anti-colon cancer targeted drug delivery

2023-05-03T21:21:17+00:00

Sunitinib malate (SM) is a multi-targeted tyrosine kinase (TK) inhibitor. It is proposed as a potent anticancer drug intended for colon cancer. The current work aimed at developing Sunitinib malate -loaded nanoparticles for colon- targeting. SM loaded ES100-nanoparticles were developed by nano-precipitation method using poly vinyl alcohol (PVA) as stabilizer. Different formulation of ESNP (F1-F3) were prepared and evaluated for particle size, poly-dispersity index, and drug entrapment, using Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), and scanning electron microscopy (SEM). The optimized formulation was further studied by in-vitro drug release and anticancer cell line study. The mean particle size, PDI, %EE of optimized SM loaded ESNPF2 was found to be 384.8±5.53 nm, 0.58, and 60.31% respectively. In vitro release of the optimized ESNPF2 showed 10.2 % drug release till 2 h, at 4h 29.6% and 12 h and 24 h 66.45%, 82.2% respectively at pH 7.4. SEM images and surface morphology confirmed that nanoparticles were spherical and had smooth surfaces. An MTT assay was conducted in the colon cancer cell lines (CACO2) to evaluate anti-cancerous activity of SM. SM loaded ESNPF2 showed a high potential against colon cancer cells. The overall results suggest that SM loaded ES100-nanoparticels could be a potential option for colon targeting.

HFE gene changes in obese individuals in Mosul

2023-01-09T15:26:54+00:00

Obesity represent one of major health problem worldwide in previous work in our laboratory significant change in iron status was notic. This study try to discover the relationship HFE gene copies number and obesity. This case control study included 90 subjects classified as 50 obese and 50 normal weight as the control group. Their ages ranged from 12 to 22 years old and carried out in Mosul during the period of April to June 2022. Complete blood picture was used to study the change in blood parameters. In addition serum ferritin was evaluated using ELISA kit.Gene copies number was assess using the GoTaq qPCR. The results of this work showed that in obese patients highest levels of hemoglobin found in compare to control (p<0.05) As observed in patients leukocyte level was which significantly higher than control p<0.05. Elevated levels of Platlate were observed in a significant higher proportion of patients than controls, Neutrophil and Lymphocyte also showed a significantly higher level in obese than control. High serum ferritin levels in obese individuals was observe in compare to controls. There was significant increase in the number of copies of the HFE gene in obese patients compare controls. In conclusion, HFE gene copies number increase in obese individuals as compensatory mechanism.

Exercise Cause Patellofemoral Pain among Young Adult: a Cross Sectional Study

2023-06-03T18:15:44+00:00

Abstract

Patellofemoral pain syndrome (PFPS) is a prevalent musculoskeletal injury affecting both athletes and non-athletes, characterized by anterior knee pain. Diagnosis can be difficult due to similar symptoms with other conditions. Treatment should focus on overall rehabilitation, including strengthening and stretching exercises, rest, ice, bracing and taping. The knee joint is complex and plays an important role in daily activities, but is also one of the most commonly injured joints.This study was conducted at the University of Erbil Technical and Medical Health College to investigate PFPS in college students aged between 20-40 years there were 102 participants. A self-report questionnaire was used to collect data on biographic information. It was found that instead of the knee pain is more among the athletic group but the result statistically insignificant predictor of knee pain among the study participants P-value = 0.823. Finally, our study concluded that athletic and non-athletic developed PFPS , but athletic more than non- athletic , male and female affected by this condition, also types of exercises have effect according to results strengthen and stretching exercise they do for example football, fitness caused PFPS.