SDS-Page Analysis Of Cysticercus tenuicollis In Vitro And In Vivo In Basrah Province/ Southern Iraq
DOI:
https://doi.org/10.59480/phahs.v1i2.26Keywords:
Capparis spinosa ; Cysticercus tenuicollis ; SDS-PAGE; Syzygium aromaticum .Abstract
The larval stage Cysticercus tenuicollis was isolated from slaughtered sheep and goats at the Basrah slaughterhouse with 30 samples. In vitro 16 samples, 2 control, and 14 were treated with plant extracts and novel chemical compounds. In vivo 14 sample,1 control and 13 treated with plant extracts and novel chemical compounds.. SDS- page electrophoresis using the gel as separation gel of larval stage Cysticercus tenuicollis. Lanes (C) control, (A) Syzygium aromaticum, (B) Capparis spinosa, novel acylselenourea, i.e., 4-Nitro-N-((4-(N-(pyrimidin-2-yl) sulfamoyl) phenyl) carbamoselenoyl) Benz amide (1), 4-Methyl-N-((4-(N-(pyrimidin-2-yl) sulfamoyl) phenyl) carbamoselenoyl) Benz amide (2), 4-Nitro-N-((4-nitrophenyl) carbamo selenoyl) Benz amide (4), N-(2,6-dioxo-1,2,3,6-tetrahydropyrimidine-1-carbonoselenoyl)-4-nitrobenzamide (5) and novel acylthiourea 4-Methyl-N-((4-(N-(pyrimidin-2-yl) sulfamoyl phenyl) carbamothioyl) Benz amide (3). The rats were administered plant extracts and synthesis compounds orally via mouth for 15 days, Then the rats were dissected, the cysts were isolated, and their SDS page was measured.The results found in in vitro, all plant extracts, and synthesized compounds bands ranged from 67 to 17KD. In contrast, the protein bands of cysts varied from 67 to 17KD in vivo whereas Capparis spinosa and the synthetic chemical (5) showed no bands. This study recorded a clear effect on protein cotenants of C. tenuicollis which was treated with two plant extracts C. spinosa and S. aromaticum, and five novel chemical synthesized compounds in vitro and in vivo by SDS- PAGE as compared with untreated.
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